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Features of reproduction of herpes viruses in conditions of mixed infection and the development of promising means of antiviral action


Work number - M 55 AWARDED

Presented by D. K. Zabolotny Institute of microbiology and virology NAS of Ukraine

 

Authors: Ph.D.Biliavska L. O.,Ph.DNaumenko K. S., Ph.DShydlovska O.A.

 

The purpose of the work is to study the features of the herpesvirusesreproduction in a mixed infection. Also, the work aimed at developing the effective antiviral preparations based on cerium dioxide nanoparticles, fluorine-containing analogs of nucleosides, nucleotides, derivatives of amino acids and determining their antiviral action.

Diseases caused by the herpes simplex virus are among the most common and poorly controlled human infections. The reason is that the virus after a primary infection persists in the human body in the nerve ganglia for life. As a result of avoiding interaction with the immune system, the herpes virus can cause acute and chronically recurrent infections, the reactivation of which can occur as a result of the co-infection with other pathogens. Investigation the patterns of the virus influence on regulatory processes in the cell during infection is the basis for understanding the pathogenesis and immunogenesis of the processes caused by the virus. The authors developed the model of adeno-herpetic infection and investigated the features of the reproduction of the associated viruses in it. It was established that under conditions of mixed infection there is a significant change in the effectiveness of the antiviral compounds, in particular, etiotropic drugs, which indicates the need for an in-depth study of the development of virus-virus interactions and the need for the use of mixed infection models for the screening of antiviral drugs.

The search for new effective drugs makes it possible to solve the problem of viruses resistance.The source of such drugs can be the nanoparticles of metals. A comparative analysis of the antiviral action of nanoparticles of metals, metal oxides, and complex nanoparticles has been carried out. Cerium dioxide nanoparticles (CDN) have as a direct effective antiviral action, as well as in nanobiocomposites with interferon, tumor necrosis factor, components of the influenza vaccine. CDN can be considered as a promising antiviral agent for the treatment of viral infections. Among the range of fluoride compounds, the authors discovered new classes of antiviral agents with pronounced anti-herpetic activity and investigated the mechanisms and targets of their action. It has been established that the fluoride compounds affect the late stages of the reproductive cycle of viruses: DNA replication, protein synthesis, and the formation of a complete and infectious viral offspring.

Thus, the presented cycle of scientific works summarizes the results of the investigation that aims at the functional patterns of the virus-cell-antiviral drug interaction on the human herpesvirus model. Obtained data of the search for specific antiviral therapy are presented. The use of new classes of chemical compounds, the technology of nanoparticles and nanocomposites is a scientific foundation for the development of drugs on their basis.

The scientific results of the work are described in 23articles in scientific journals (12 foreign), and 1 patent. According to the Scopus database, the total number of references to the authors' publications presented in the work is 12, the h-index (at work) = 2; According to the Google Scholar database, the total number of links is 18, the h-index (at work) = 3.

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