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Optimization of early diagnosis of non-alcoholic fatty liver disease


Presented Institution "L.T. Malaya National Institute of Therapy of the NAMS of Ukraine".

Cherelyuk N.I. junior researcher of the department of study of diseases of the digestive system and their comorbidity with non-infectious diseases of the State Institution "L.T. Malaya National Institute of Therapy of the NAMS of Ukraine".

Goal of the study. To optimize the early diagnosis of fibrosis in patients with NAFLD on the background of obesity and overweight by studying the ratio features between phylotypes Firmicutes / Bacteroidetes of GM, molecular genetic factors: microRNA-122, microRNA-34a, and proinflammatory markers: high-sensitivity HSRP and TNF-alpha.

Materials and methods.

To achieve the goal and objectives, the prospective study was developed, which included 140 patients. The main group consisted of 120 patients who were divided into two subgroups depending on BMI and 20 practically healthy volunteers, corresponding in age and sex.

The next stage of the study was the analysis of the clinical manifestation of NAFLD, followed by determination of the composition of GM, for which the relative content of the most common phylotypes of GM was analysed.

To clarify the pathogenetic mechanisms through which GM can affect the formation of clinical syndrome complexes in the studied patients, the content of proinflammatory and profibrotic markers - HSRP, TNF-alpha, microRNA-34a and microRNA-122 was additionally studied.

To achieve the goal of the study, the last stage was to develop the four-stage algorithm for screening fibrosis in patients with NAFLD on the background of obesity and overweight.

Results and conclusions:

1. Changes in the composition of GM in patients with NAFLD were characterized by the increase in the relative content of Firmicutes against the background of the decrease in the relative content of Bacteroidetes, which was more pronounced in patients of the first subgroup.

2. In patients with NAFLD, there was the increase in serum concentrations of miRNA-122 in the presence of excess weight in 40 times, and in concomitant obesity - in 39.09 times; increase in miRNA-34a by 6.20 times and 6.40 times, respectively.

3. In patients with NAFLD, miRNA-122 was positively correlated with Firmicutes / Bacteroidetes ratio in patients in both subgroups and negatively with Bacteroidetes in overweight patients; miRNA-34a was negatively correlated with Bacteroidetes and positively correlated with the Firmicutes / Bacteroidetes ratio in overweight patients.

Number of publications: 18 scientific papers were published, including 7 articles, one of which is in English, 2 articles - in foreign publications, including in the journal indexed by the scientometric database Scopus, h-index -1. 2 utility model patents were received.