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Microbial and molecular genetic biotechnologies for preservation, bioremediation and increasing of agroecosystems productivity

Work number - M 39 FILED

Presented D.K. Zabolotny Institute of Microbiology an Virology of the NAS of Ukraine

Zlatogurska М.А., Loboda M.I., Havryliuk О.А., Dimova M.I.

Extensive use of organochlorine and metal-containing pesticides increasingly disrupting the functioning of ecosystems promotes the accumulation of toxic compounds in soils. This actual environmental problem requires the development of effective problem-solving approaches. Among others is the implementation of microbial biotechnologies for treatment and productivity restoration of depleted and contaminated soils.

The work aimed to develop scientific bases and implement microbial and molecular genetic biotechnologies for preservation as well as increasing productivity of modern agroecosystems under anthropogenic impact.

Microbial community resistance to organochlorine contamination was evaluated for main soil types in Ukraine for the first time. Two organochlorine resistant strains of Comamonas testosterone ‒ promising hexachlorobenzene destructors ‒ were isolated and identified. It wat established that the C. testosteroni 46 culture fluid has a positive effect on maize growth features under soil pollution.

Relying on thermodynamic prognosis the theoretical bases for the optimal bioremoval ways of toxic copper from solutions and soils were described. For the first time, the copper(II) high resistant strains as well as the cadmium resistant strains were isolated and identified. Further analysis of their genomes also revealed genetic determinants of copper and cadmium resistance.

New aspects of the biosynthetic ability of the Streptomyces netropsis IIA Ac-5025 were studied. It was shown that this bacteria produces a polyene antibiotic complex effective against a number of phytopathogenic fungi and bacteria. The obtained data founds the perspective of streptomycetes metabolites usage for plant adaptation, growth regulation and phytoprotection.

Part of the work was also devoted to the study of genome organization of temperate phages 49 and 59 Erwinia horticola. These novel members of the lambdoid phage group are unique as their genomes reveal no significant similarity to that of any previously reported viruses. A number of genes involved in host resistance formation were identified in their genomes. Furthermore, the information on virion DNA of phages 49 and 59 has been refined and supplemented. The hypothetical model of new phenomenon named phage mediated phage induction was proposed.

Number of publications: including 16 articles in professional journals (1 paper is in international English-language journals with impact factor), 1 methodical recommendation and 44 abstracts on Ukrainian and international conferences. The number of valid patents of Ukraine: 2 (1 patent for an invention and 1 patent for a utility model). The total number of references to the authors' publications presented in the work is: 1 (Web of Science), 4 (Scopus), 10 (Google Scholar). h-index: 1 (Web of Science), 1 (Scopus), 3 (Google Scholar).