Work number - M 43 FILED
Komok M.S., Pirog A.V..
Work is devoted to the optimization of Bradyrhizobium japonicum M 8 interaction with physiologically active substances of vermicompost during the inoculation of soybean and the improvement of Rizogumin production process, to the research of the Rizogumin usage efficiency in the technology of soybean and yellow lupine growth. Also in the paper the influence of a complex action biological preparation on the flow of a viral infection in the yellow lupine plants was studied.
Using the quantitative methods of the spectrodensimetrical thin layerchromatography (TLC) and ELISA it has been investigated the auxin and cytokinin content in the components of a biological preparation Rizogumin. It has been shown that the optimal phytohormonal procuring for the formation and functioning of the soybean plants symbiotic system is: auxin - 1,46 × 10-3g/ seed, cytokinins -3,3 × 10-4g/ seed. This phytohormone weight is recommended for the Rizogumin production.
The influence of the different microbial preparations on the introduction of the industrial strain into the agrocenosises has been defined. During the comparing of the inoculants influence to the activity of introduction impact of the B. japonicumM 8 strain it has been determined that the biological preparation Rizogumin provided the formation of the greatest number of the nodules formed with the participation of the specified strain.
It has been found that among all investigated preparations Rizogumin effected the most actively on the formation and functioning of nitrogen-fixing symbiosis, plant growth, also stimulated the physiological processes both in soybean and yellow lupine. The using of the biological preparation of complex action ensured the increasing of the soybean yield by 33 - 61 % , the grain yield and the green mass of yellow lupine by 28% and 41% .
The using of the complex preparation Rizogumin contributed the reduce of the virus concentration in lupine plants by 19.0 - 26.6 %. Under the influence of the complex inoculum it was being noted the increase of the endogenous phytohormones content, both in healthy and diseased with the viral infection plants of yellow lupine, which explains the activation of the physiological processes in the plants. The action of the complex biological preparation provided the growth of the grain yield and the green mass of the infected plants by 53.8 % and 52.7 %.
Based onthe research, it has been published 29papersincluding, 1 scientific monograph,10articlesin the professional journals,3articles,15abstractsin national and international conferences proceedings.